Plants produce a diverse range of bioactive molecules, making them rich source of different types of medicines. In the present study the, In vitro antibacterial activity of 3 medicinal plants viz., Parthenium hysterophorus, Stevia rebaudiana and Ginkgo biloba were evaluated by using different solvent extracts. The extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis, Enterococcus spp., and Staphylococcus aureus by using agar well diffusion method. Azithromycin and cepaxim were used as standard antibiotics. Some of the solvent extracts of the plant showed the highest activity against some bacteria than standard antibiotics used. The findings provide support for the use of these plants in traditional medicine.