OBJECTIVE: To study the possible anti-inflammatory effect of CUMANDA, measured by edemas, induced by carragenin in the feet of laboratory mice.
BACKGROUND: The method of inducing edemas by applying carragenine to the feet of mice is a classic model for the study of products with anti-inflammatory activity. The byproducts of the metabolism of araquidonic acid via cecloxigenesis and the production of reactive species of oxygen are also involved. It is reported that there are four principle phases to this edema: an initial phase in which histamine and serotonin is released; a second phase measured by kinins; a third phase (about 5 hours) in which prostaglandins are liberated; and a fourth phase related to the local infiltration of neutrophils and their activiation. This model has recently been recommended as very useful for the evaluation of anti-oxidant products with anti-inflammatory properties and free radicals of oxygen inhibitors.