Background: Oxidative radicals are major environmental causes of human skin damage. Oxidative defense factors, in-cluding nuclear factor erythroid-derived 2-related factor 2 (Nrf2), are centrally involved in repairing skin cells or protecting them from oxidative damage. Coriandrum sativum L. (co-riander; CS) is a commonly consumed food and a traditional phytomedicine in Asia and Europe. In this study, we examined the protective effects of a standardized CS leaf extract against oxidative stress in human HaCaT keratinocytes.
Methods and Results: CS significantly and dose-depend-ently protected cells against reduced cell viability caused by H 2 O 2 -induced damage, as assessed using the 3-(4,5-dimeth-ylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Oth-er assays demonstrated that CS protected HaCaT cells by in-creasing the levels of glutathione and activities of oxidative defense enzymes, such as superoxide dismutase and cata-lase. Moreover, it increased the expression of activated Nrf2, which plays a crucial role in protecting skin cells against oxi-dative stress.
Conclusion: These results suggest that CS protects human keratinocytes from H2 O2 induced oxidative stress through antioxidant effects.