Liver is the ﬁrst organ to metabolise all foreign compounds and hence it is susceptible to almost as many different diseases. Some are rare but there are a few, including hepatitis, cirrhosis, alcohol-related disorders and liver cancer. A major cause of these disorders is due to exposure to different environmental pollutants and xeno-biotics e.g., paracetamol, carbon tetrachloride, thioacetamide, alco-hol, etc. These toxicants mainly damage liver by producing reactive oxygen species (ROS).
Reactive oxygen free radicals have been known to produce tis-sue injury through covalent binding and lipid peroxidation and have been shown to augment ﬁbrosis as seen from increased colla-gen synthesis (Geesin et al., 1990). Scavenging of free radicals by antioxidants could reduce the ﬁbrosis process in the tissues (Thre-siamma and Kuttan, 1996). Free radicals may also be a contributory factor in a progressive decline in the function of immune system (Pike and Chandra, 1995). Cooperative defense systems that pro-tect the body from free radical damage include the antioxidant nutrients and enzymes. The antioxidant enzymes include superox-ide dismutase (SOD), catalase (CAT) and glutathione peroxidase(GPx). Their role as protective enzymes is well-known and has been investigated extensively with in vivo models.