The aim of present study was to investigate the wound healing activity of the Indian medicinal plant Achillea millefolium L. The alcoholic and aqueous extracts of leaves of Achillea millefolium L was studied for its effect on wound healing in Wistar rats, using incision, excision, and dead space model at dose levels of 200mg/kg. Alcoholic and aqueous extracts of leaves of Achillea millefolium L. showed a definite, positive effect on wound healing with a significant increase in the rate of wound contraction, skin breaking strength, granulation tissue dry weight and wet weight content, and breaking strength of granulation tissue. In histopathological studies showed increased collagen when compared to the control. The efficacy of Achillea millefolium L. in wound healing may be due to the presence of active principles, which accelerate the healing process and confers breaking strength to the healed wound. So it is possible to conclude that, this plant has wound healing activity and there by justifying traditional claim.