Introduction: Hyssopus officinalis L. is a well-known herb for its culinary and medicinal significance. The purpose of this study was to perform the pharmacognostic evaluation.
Methods:Physicochemical and phytochemical analysis, HPTLC quantification and in vitro antioxidantand antidiabetic activity were done.
Results: Preliminary screening revealed the presence of phytomolecules such as alkaloid (0.99%), tannin (1.75%), sugar (1.96%) and starch (0.68%).Total phenolic and flavonoid content were found to be 2.32% and 1.16% respectively. HPTLC quantification data showed that the content of ferulic acid (0.034%) was higher than caffeic acid (0.0064%) on dry weight basis The IC50 value for the in vitro DPPH radical scavenging assay was 0.50 µg/ml and in vitro anti diabetic assay displayed IC50 value of 0.8366 mg/ml.
Conclusion: The study suggests presence of considerable amount of phenolic acids andantioxidant activity in the plant which supports its use in the traditional systems of medicine