In this present investigation, AgNPs were green synthesised using Coriandrum sativum leaf extract. The physicochemical properties of AgNPs were characterised using UV–visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray (FESEM/EDX), Fourier transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Brunauer-Emmett-Teller (BET) analysis. Further, in vitro anti-acne, anti-dandruff and antibreast cancer efficacy of green synthesised AgNPs were assessed against Propionibacterium acnes MTCC 1951,Malassezia furfur MTCC 1374 and human breast adenocarcinoma (MCF-7) cell line, respectively. The flavonoids present in the plant extract were responsible for the AgNPs synthesis. The green synthesised nano particles size was found to be ≈37 nm. The BET analysis result shows that the surface area of the synthesised AgNPs was found to be 33.72 m2 g−1. The minimal inhibitory concentration (MIC) of AgNPs for acne causative agent P.acnes and dandruff causative agent M. furfur was found to be at 3.1 and 25 μg mL−1, respectively. The half maximal inhibitory concentration (IC50) value of the AgNPs for MCF-7 cells was calculated as 30.5 μg mL−1 and complete inhibition was observed at a concentration of 100 μg mL−1. Finally, our results proved that green synthesised AgNPs using C. sativum have great potential in biomedical applications such as anti-acne, anti-dandruff and anti-breast cancer treatment.